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Vývoj molekulárních markerů pro chromozom W u modráska \kur{Polyommatus icarus}
HRUBÁ, Monika
This thesis focuses on development of molecular markers for the W chromosome in the blue butterfly Polyommatus icarus. In this blue butterfly, karyotype races with different constitution of the neo-sex chromosomes were reported. The markers were used for PCR sexing of the early developmental stages to obtain sex-specific total RNA samples for testing a role of sexually antagonistic selection in fixation of neo-sex chromosomes.
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Analýza repetic v genomech vybraných druhů modrásků rodů \kur{Polyommatus} a \kur{Lysandra}
HRUBÁ, Monika
This thesis focuses on the analysis of mobile elements in the genera Polyommatus and Lysandra (Lepidoptera) with a potential impact on karyotype fragmentation. The presence of selected mobile elements in genomes of 15 lycaenid species was tested by PCR. Moreover, the same method was used to detect these elements in 13 selected ant species, which may present a source for lateral gene transfer in myrmecophilous blue butterfly species. Selected transposable elements were localized in pachytene nuclei using fluorescence in situ hybridization. The results of this thesis suggest a patchy phylogenetic pattern of studied repeats which can be partly explained by mobile elements spread through interspecific hybridization and horizontal gene transfer among studied Polyommatus and Lysandra species.
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The influences of biotic interactions on population biology of Sanguisorba officinalis
Větvičková, Anna ; Janovský, Zdeněk (advisor) ; Jičínská, Jana (referee)
Plants can interact with many different organisms on many trophic levels in their life. In this thesis I investigated how three biotic inertactions, powdery mildew infection, caused by Podosphaera ferruginea, predispersal seed predation by Dusky Large Blue (Phengaris nausithous) and Scarce Large Blue (P. teleius) and deer grazing affect life cycle and population biology of the Great Burnet (Sanguisorba officinalis L.). I also analysed their preferences of environmental conditions. Most of studied plant populations growth rates (λ) were lower than 1 and population decreased, but quite surprisingly for more populations vegetative reproduction was more important than reproduction by seeds. All intraction mentioned above affects more generative reproduction, and thus their influence on Great Burnet population dynamics is low, even with modelled maximal possible effect. Environment conditions analysis showed that powdery mildew occurs on sites with more flowering individuals, where spreading is very easy, butterflies preferred high, well noticeable flower heads grown high above other vegetation and host ant presence. For host ants' presence model locality was only significant variable.
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The role of insect herbivory in plant population dynamics - a case study of the Lycaenidae family
Barusová, Anna ; Janovský, Zdeněk (advisor) ; Sedláček, Ondřej (referee)
Herbivory is one of the key interactions of plants, which can result in important consequences for their population dynamics and which can also exert selection pressure on evolution of their traits. This bachelor thesis is concerned with insect herbivore influence on plants at the levels of individuals and population dynamics. It also focuses on interactions of occurrence of herbivory with environmental conditions. This is demonstrated on the family Lycaenidae (Lepidoptera) which is highly specialized in terms of host plants with several cases of coevolution having been described. Some Lycaenid species also evolved associations with ants probably, which help to increase their range of host plants. The relationship of feeding strategies and myrmecophily is illustrated on Czech species of Lycaenidae. The last part focuses on the Great Burnet (Sanguisorba officinalis) and its European-wide declining monophagous herbivore Dusky Large Blue (Phengaris nausithous), their ecology and conservation. Ecology of this study system will be the main topic of the intended master thesis.
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Genetic diversity of Czech populations of critically endangered blues (Lepidoptera, Lycaenidae)
BENEŠ, Jiří
Butterfly family Lycaenidae is cosmopolitan. Some species of the family are common in the Czech Republic, but some are at the risk of extinction, which is caused either by lack of habitat or host plant deficiency. Among the most endangered species in the Czech Republic are the damon blue (Polyommatus damon (Denis a Schiffermüller, 1775)) and the turquoise blue (Polyommatus dorylas (Denis a Schiffermüller, 1775)). This thesis aims to review the biology of both species, causes of threat and possibilities of their practical conservation, and to describe genetic variability of Czech populations of two critically endangered Lycenids, the damon blue and the turquoise blue, and to compare them with European populations for potential import of alien individuals to Czech populations. Genetic assignment is important for possible reintroduction or revitalization of Czech populations from foreign sources. I detected in this thesis that the damon blue has more genetic variability than the turquoise blue and this pattern is not only geographical. I assume that Czech populations of both species experienced bottleneck and are under genetic drift. Furthermore, the Central European individuals genetically differ from Southern European and Baltic samples, and the number of haplotypes is high in Central Europe. The current distribution is not caused by colonization after the last glacial period. According to the results, it is appropriate to protect all current populations separately, rather than supplement them from other sources, because they can be genetically different. Finally, I discuss the management planned for the two species in the Czech Republic and connect it to my results.
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Comparison of population parameters of Large blues from genus Phengaris on multiannual basis
Bártů, Nikola ; Vrabec, Vladimír (advisor) ; Petr, Petr (referee)
There where evaluated the changes in abundance of overall metapopulations and abundace of butterflies on surveyed areas occupied by Phengaris nausithous (Lepidoptera: Lycaenidae). It ran on two long-term monitored locations in the Czech Republic : Slavíkovy ostrovy u Přelouče a Dolní Labe u Děčína. Data for this comparison was obtained by longterm monitoring of the abundance of butterflies on these locations. The method of reverse capture of labeled specimens was used and then statisticall processing of marking results in program MARK. In final number of the target species has been found significant fluctuation , after our evaluation. Abudance of total metapopulation in Přelouč in years from 2004 till 2016is variable from 148 up to 1 369 butterflies, numbers in Děčín between 2008 and 2016 has vacillated from 273 to 1952 individuals. Growth of abudance of metapopulation on these two locations are obvious from crossing both diagrams (in years 2006, 2010-11 and 2014-2015). However, observing time-lime is not so long, this is very strong argument to support this hypothesis: ,,From aged time sequence there is noticable graduation periodicity. Than has been compared progression of population of individual locations compare to metapopulation of both locations together and it has discovered this observation is a real picture of aged progress. Most of places of research copying trend of metapopulation. If there are any differences, for example total metapopulation grows, but population of individual location declines, it could be explain as a change of familiar location related on mamagement, in this case on the wrong one. Contrariwise, rising of individual colony against the trend of metapopulation, mostly shows a positive changes in maintenance of site. Long therm researching is highly recommented for rating of impact of management.
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The status of Phengaris nausithous population on selected meadows in Broumovsko region
Štěpánková, Anežka ; Vrabec, Vladimír (advisor) ; Kamil, Kamil (referee)
The purpose of this thesis entitled Population Status checkered blue butterfly Phengaris nausithous (Bergsträsser, 1779) on selected meadows in Broumov area was to find and evaluate the situation regarding the size and structure of populations of the butterfly Phengaris nausithous, on a recently selected location within the territory of the Czech Republic. A used monitoring method was a Capture-Recapture method at 13 locations in the PLA Broumovsko, where the occurrence of the food plant Sanguisorba officinalis, which is an integral part of the species evolution, was the key for choosing the location. The ant of the genus Myrmica is also important for the evolution of the P. nausithous. The results were evaluated using the program MARK and Statistika and then transferred into graphs and tables. In 2016 it was marked 687 of the P. nausithous and the sum for the overall metapopulation totaled 1,487 individuals. Most of the P. nausithous occurence with the number of (fixed catches) 149 was registered at the Hynčice 2 (H2) area, which was the third largest area of all studied habitats. The least number of 15 of the P. nausithous has been marked at the Hynčice 1 (H1) area. Maximum estimates of numbersfor patches were determined with the program MARK with the highest number of 367 individuals in the H2 area, whereas the lowest number of 38 individuals at the H1 area.The numbers of P. nausithous determined by the application MARK were significantly higher than those of fixed catches, indicating good aplication of methodology. It was captured a total of 49 migrations. Overall 31 % of individuals migrated and 18 % of individuals migrated within assumed total number. Most flyovers were between the areas H2 and H4 (11 flyovers) and between areas V1 and H4 was recorded flyover at a distance of 5.3 km, which can be considered as one of the longest flyovers. The average distance of migration of P. nausithous was 1.46 km. The number of immigrants for solid flyovers was set to 49 individuals and the number of immigrants to 58 individuals. The hypothesis of this work was: All the examined areas communicates using flyovers of butterflies and the system can be considered as a single metapopulation and was confirmed given on the basis of the results. Target species dependence on the density of host plant vegetation was not crucial according to the graphic representation. The first T-test found that there is no association between P. nausithous abundance and between the length of the migration between particular areas. The second T-test found that with increasing number of flyovers the length of those flyovers gradually decreased.
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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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